Disulfide Bond Analysis

Figure 1 Peptide cyclization through a disulfide bond
for multiple purposes¹.Disulfide bond analysis is a key part of the biopharmaceutical process, such as antibodies, peptides and other protein drugs. Disulfide bond is a covalent bond between sulfur atoms formed by oxidation of sulfhydryl groups on two cysteine. Disulfide bond plays an important role in forming stable protein structure, maintaining correct conformation and regulating biological activity. Disulfide mismatch or incomplete formation of disulfide bonds often implies a decrease in the biological activity of the drug. Therefore, the disulfide bond pairing in the whole biopharmaceutical R & D process should be monitored, such as cell clone screening, purification process validation, formulation screening, product stability confirmation and so on.

With Alfa Chemistry's cutting-edge analytical technology, complex disulfide bond analysis, be it normal disulfide bonds, closely spaced disulfide bonds, or mismatched disulfide bonds, is available to customers worldwide. We have experienced scientists working with you to develop tailor-made analytical solutions. Alfa Chemistry provides one-stop disulfide bond analysis services, including sample preparation, protein purification, enzymatic hydrolysis, LC-MS/MS identification, bioinformatics analysis. You only need to send samples to us, communicate your research objectives with our experts, we will complete the next steps for you and provide a complete technical report. For any questions about disulfide bond analysis, please feel free to contact us.

Alfa Chemistry's capabilities include but not limited to:

• Analysis of unknown S-S bond connections in proteins.

• Analysis and prediction of whether proteins are folded correctly.

• Verify that the disulfide bonds in the protein are mismatched.

• Identification of proteins, providing disulfide bonds mapping.

• Bioinformatics analysis.

Alfa Chemistry's technology platforms include but not limited to:

• Two-dimensional Difference Gel Electrophoresis (2-D DIGE)

• Hydrophobic Interaction Chromatography (HIC)

• Capillary Electrophoresis (CE)

• Fourier Transform Ion Cyclotron Resonance-Mass Spectrometry (FTICR-MS)

• MALDI-TOF-MS

• ESI-QTOF-MS

Alfa Chemistry's advantages:

Reference

• Miriam Gongora-Ben ítez. (2014) 'Multifaceted Roles of Disulfide Bonds. Peptides as Therapeutics.' Chem. Rev. 114 (2): 901–926.