Plant Tissue Testing
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Note: this service is for Research Use Only and Not intended for clinical use.
Alfa Chemistry offers comprehensive phytotrophic tissue solutions for assessing potential deficiency symptoms in plants. Our plant tissue testing service provides valuable insights into the nutrient status of plants, helping growers optimize their fertilization strategies and maximize crop yields.
Why Sample Plant Tissue or Grains?
Sampling plant tissue or grains is crucial for understanding the nutritional needs of plants. By analyzing the elemental composition of plant tissues, we can identify nutrient deficiencies or imbalances that may adversely affect plant health and productivity. Plant tissue testing allows for targeted and precise fertilizer recommendations, leading to improved crop quality and increased profitability.
Alfa Chemistry's Plant Tissue Analysis Project
We offer a comprehensive range of plant tissue element analysis options to meet the specific requirements of our clients. Our testing methods provide accurate and reliable results, enabling growers to make informed decisions about their fertilization practices. The following analysis options are available:
Standard Plant Analysis Kit
Our standard plant analysis kit covers a wide range of essential plant nutrients. It includes the following elements:
- Nitrogen (N)
- Phosphorus (P)
- Potassium (K)
- Calcium (Ca)
- Magnesium (Mg)
- Manganese (Mn)
- Iron (Fe)
- Copper (Cu)
- Boron (B)
- Aluminum (Al)
- Zinc (Zn)
- Sodium (Na)
- Sulfur (S)
Separate Analysis
We also offer separate analysis options for specific elements:
- Complete - Acid Digestion - Sample digestion and ICP analysis: N, P, K, Ca, Mg, Mn, Fe, Cu, B, Al, Zn, Na, S
- Standard - Acid Digestion - Sample digestion and ICP analysis: P, K, Ca, Mg, Mn, Fe, Cu, B, Al, Zn, Na, S
- Trace - Acid Digestion - Sample digestion and ICP analysis: Cd, Co, Pb, Mo, Ni
Additionally, we provide the following analysis services:
- Nitrogen: Total nitrogen analysis using dry combustion analysis
- Carbon: Total carbon analysis using dry combustion analysis
Sampling Instructions for Plant Analysis
Ensure that each sample has a quantity of plant tissue leaves or at least 10 g of dried plant tissue material. A large handful of threshed grains/seeds in a self-sealing bag will suffice. You will need to fill in the relevant sample information on the Plant Tissue Submission Form we provide. We recommend placing all paperwork in a clear plastic bag. Place the submission form and sample in a shipping bag, seal it, and deliver it to us.
If you choose to take your samples, we will provide clear instructions on how to collect plant tissue samples or grains for analysis. These instructions cover sample size, sampling locations, sample handling, and packaging.
Our Plant Tissue Analysis Technology
Sample digestion and ICP analysis is a crucial steps in plant tissue testing. It involves the preparation of the plant tissue samples for elemental analysis using the technique of acid digestion, followed by ICP analysis to measure the elemental composition of the sample.
During the sample digestion process, the plant tissue samples are carefully prepared and digested using appropriate acid solutions. Acid digestion helps break down the organic matter and release the elements of interest from the sample matrix, making them available for analysis. The digestion process ensures that the elements are in a form that can be accurately quantified using analytical techniques.
After digestion, the resulting solution is then subjected to ICP analysis, which allows for simultaneous multi-element analysis, providing rapid and accurate results for a wide range of elements.
Fig. 2 Plant analysis by ICP OES using microwave-assisted digestion using dilute acid and alkaline solutions. (Barros JAVA, et al. 2016)
Contact us today to learn more about how our plant tissue testing services can benefit your farming operation.
Techniques for Plant Tissue Testing
Growth Chamber
The growth chamber, also called a plant culture chamber, is one of the indispensable equipment for plant experiments. The growth chamber can provide an environment suitable for plant growth, and control parameters such as light, temperature, and humidity, providing researchers with a controllable experimental environment.
Microscope
The microscope is also an essential instrument for plant experiments. The microscope can provide high-magnification lenses, helping researchers observe small details such as anatomical structures and cell structures. In the study of plant leaf anatomy and pathology, the microscope plays a vital role.
Centrifuge
The centrifuge is an indispensable equipment in the laboratory, and plant experiments are no exception. The centrifuge can separate plant tissues, cells, and other samples by centrifugation, thereby obtaining clear sample separation liquids and supernatants. This is very important for plant physiology, biochemistry research, and gene separation research.
Electrophoresis Device
The electrophoresis device is a device that separates biomolecules through the force of an electric field. In plant genetic engineering, the electrophoresis device is often used for DNA analysis and identification of PCR amplification products. The electrophoresis device not only improves the efficiency of the experiment but also improves the accuracy of the experiment.
Photosynthesis Analyzer
The photosynthesis analyzer is also one of the common equipment used in plant experiments. The photosynthesis analyzer can measure parameters such as photosynthetic rate, respiration rate, and transpiration rate, providing researchers with rich data to analyze photosynthesis and plant growth status.
Industry articles
Determination of Hydrogen Peroxide in Plant Tissues
Junglee, S., Urban, L., Sallanon, H., & Lopez-Lauri, F. (2014). American Journal of Analytical Chemistry, 5(11), 730.
Hydrogen peroxide (H2O2) is commonly produced in the normal physiological processes and stress conditions of plants. The quantitative determination of hydrogen peroxide is important in many studies, as H2O2 is involved in oxidative cell damage and signal transduction processes.
When hydrogen peroxide is added to a colorless potassium iodide (KI) solution, the iodide ions (I-) are slowly oxidized to iodine (I2). In the presence of iodide, the iodine reacts to form tri-iodide (I3-), producing a pale yellow solution. Therefore, the quantification of hydrogen peroxide can be achieved by spectrophotometry by tracking the absorbance.
Determination of Brassinosteroids in Plant Tissues
Ding, J., Wu, J. H., Liu, J. F., Yuan, B. F., & Feng, Y. Q. (2014). Plant Methods, 10, 1-11.
Brassinosteroids (BRs) are an important class of plant hormones that have a significant impact on plant growth and development. To fully elucidate the functions of BRs, a sensitive BR determination method is required.
Here, an analysis method based on magnetic solid-phase extraction (MSPE) combined with in-situ derivatization (ISD) for BR analysis was established. TiO2-coated magnetic hollow mesoporous silica spheres (TiO2/MHMSS) were used as the micro-extraction adsorbent and "micro-reactor" for capturing and derivatizing BRs.
The MSPE-ISD method for determining endogenous BRs is rapid and sensitive. It can be used to analyze endogenous BRs in 100 mg of fresh plant tissue (Brassica napus L. (B. napus L)). The proposed plant sample preparation strategy can be extended to develop analysis methods for other analytes with poor MS response in complex sample matrices.
Reference
- Barros JAVA, et al. (2016). "Microwave-assisted Digestion Using Diluted Acid and Base Solutions for Plant Analysis by ICP OES." Journal of Analytical Atomic Spectrometry, 31, 337-343.
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