N- and C-terminal Sequencing

At any stage of biopharmaceutical development, N- and C-terminal sequencing can give a lot of information about proteins and antibodies, and demonstrate consistency and comparability between batches. Especially in the process of R & D and process establishment of recombinant protein products, it is necessary to analyze the N- and C-terminal sequences of the recombinant protein in order to confirm whether the recombinant protein is fully expressed and to detect whether or not the recombinant protein breaks during the expression process and whether the N- and C-terminal sequences of the recombinant protein were modified. In addition, ICH Q6B requires an overview of the N- and C-terminal amino acid sequences of the drug for biological manufacturers. Therefore, N- and C-terminal sequencing is one of the indispensable detection items of biopharmaceuticals.

With years of experience in the biopharmaceutical field, Alfa Chemistry provides a high level of quality control for your natural or recombinant proteins, and we can help you sequence the N- and C-terminus to confirm the properties and quality of proteins and to find cleavage sites and even to identify unknown proteins. Using the classic Edman degradation method combined with advanced mass spectrometry technology, Alfa Chemistry provides you with one-stop N- and C-terminal sequencing services to provide you with a detailed technical report, and all you need to do is send your sample to us. If you have any questions about sequencing, please feel free to contact our experts.

Alfa Chemistry's capabilities include but not limited to:

• The verification of protein expression products, antibody drugs, vaccines

• Establishment of cell lines and verification of products protein expression during fermentation

• The analysis of protein degradation / enzyme digestion

• De novo sequencing

• Other peptide & protein drug analysis

Alfa Chemistry's technology platforms include but not limited to:

• Two-dimensional Difference Gel Electrophoresis (2-D DIGE)

• Hydrophobic Interaction Chromatography (HIC)

• Capillary Electrophoresis (CE)

• Fourier Transform Ion Cyclotron Resonance-Mass Spectrometry (FTICR-MS)

• MALDI-TOF-MS

• ESI-QTOF-MS

Alfa Chemistry's advantages: