Chromatographic Techniques

Chromatography is an important and commonly used technique in the discovery and development of drugs that separates, identifies and purifies the components of a mixture and can be used for both qualitative and quantitative analysis. For example, column chromatography is one of the most commonly used methods for protein purification. Stationary phase, mobile phase, the type of interaction between the material contained in the mixture and the stationary phase form the basis of chromatographic techniques.

Alfa Chemistry's pharmaceutical analytical laboratories have extensive experience in the selection and use of chromatographic techniques, choosing different chromatographic methods depending on the sample. As a trusted third-party testing company, Alfa Chemistry provides you with the most cost-effective separation and purification services and accurate and repeatable results according to your needs. If you have any questions during drug discovery and research, please feel free to contact us.

Table 1 Classification of Chromatographic Technique based on various parameters¹

Alfa Chemistry's capabilities include but not limited to:

• Thin Layer Chromatography (TLC). Since the particle size of the stationary phase is much smaller than that of paper chromatography, the result is highly reproducible and the separation effect is more effective.

• Ion Exchange Chromatography (IEC). The principle is based on the electrostatic interaction between charged protein groups and solid support materials.

• Gel Permeation Chromatography (GPC). Separation of macromolecules from samples based on molecular weight differences, is often used to determine the molecular weight of proteins and to reduce the salt concentration of protein solutions.

• Gas Chromatography (GC). With gas as mobile phase, it is suitable for heat-resistant and volatile organic substances, and is an efficient separation method in the pharmaceutical industry.

• High Performance Liquid Chromatography (HPLC). This method is similar to GC, except that a liquid is used as the mobile phase to make up for the shortcomings of GC, which is suitable for thermally unstable organic substances such as amino acids, proteins, and hydrocarbons.

Alfa Chemistry's advantages:

Reference

• Manisha Chavan (2013) 'Significance of Various Chromatographic Techniques in Drug Discovery and Development.' IJRPC. 3(2): 2231-2781.