PacBio RS II

The PacBio RS II system conducts, monitors and analyzes single molecule, realtime (SMRT™) sequencing reactions. It uses polymerase not for amplification of clusters, but for the detection of fluorescence at a single-molecule level which are taken as video and then converted into sequences. It has the longest read length (averaged 20kb) among all sequencers on the market at the moment, which gives unparalleled advantage over the second-generation sequencers with shorter read lengths.

Applications

• Whole Genome Sequencing

• Complex Populations

• RNA Sequencing

• Epigenetics detection

• Targeted Sequencing

Strengths

• Fast sequencing: Completion of library construction and sequencing within 48 hours

• sequence Length: The average length of reading can reach 7.9 kb, the maximum length can reach KB (20kb Library, P5+C3)

• Precision is very high: >99.999%

• Good representative: no obvious GC or at preference. Flexible mode: 1-16 SMRT cell sequencing can be flexibly arranged

• multiple samples: A barcode system has been established and multiple samples are sequenced simultaneously

• Single molecule sequencing: There was no PCR amplification during the construction of the library, and a rare mutation could be detected

• Real-time sequencing: The basic modification information can be obtained directly during the sequencing process

• gap Few: Genome <3m sample, Gap number 0-10

• Widely used: eukaryotic and prokaryotic genomes, PCR products, full-length cDNA sequencing, genome-wide methylation and other sequencing

• Automated consumable handling with integrated software

• Intuitive run setup and monitoring

• Run-time flexibility up to 20 hours per SMRT Cell

• Walk away time up to 4 days

• Absolute run size flexibility

• Intelligent run progress monitoring