Microbiology Testing

Pathogenic microorganism in food is the most important biological factor affecting food safety. It is highly likely to cause widespread disease. The vast majority of foodborne diseases are caused by a small number of pathogens, including bacteria, viruses, fungi, rickettsia, parasites, and insects. Therefore, it is very important to conduct microbiological detection of food, especially the qualitative detection of pathogens. Scientists at Alfa Chemistry have mastered the qualitative testing of pathogens, including microbial culture, immunology, molecular biology, and synthesis. Some methods can be tested in a very short period of time, and both sensitivity and specificity can meet international standards.

As an expert in these fields, Alfa Chemistry provides reliable and high quality product packages to meet all the food testing requirements of your company. We are smart and use convenient solutions to bring you a lot of benefits and optimize your process.

We have a series of traditional and rapid pathogens detection solutions covering already known pathogen and new pathogens (such as e. coli O157), in addition, we also see demand constantly added new members into our product series. Alfa Chemistry ' corruption detection solutions cover typical contaminants in beverages such as beer, wine and fruit juice.

Microbial toxins include bacterial toxins, endotoxins and toxins. However, due to the microbial toxins harm to human body, a lot of bacterial toxins heat resistance is strong, despite the heating mode that can kill microorganisms, but not necessarily can make the toxin and deactivation. So to strengthen the detection of bacterial toxins in food is very necessary and important, especially for quickly finding pathogens in the food poisoning. At present, the method of detection of bacterial toxins in food is mainly based on the antigen antibody specificity of immune reaction AGAR diffusion method, reverse indirect hemagglutination test, radioimmunity method, immunofluorescence, enzyme-linked immunosorbent method, the article jinbiao reagent, and immune biosensor method such as immunological method; Animal experiments can also be used to determine the levels of bacterial toxins in food, both of which directly measure bacterial toxins in food. Alfa Chemistry has advanced detection equipment and professional testing team, which can detect common microbial toxins such as aflatoxin, voltamoxin, and ochratoxin. At the same time, microbial detection method can be used to determine whether food is contaminated by pathogenic microorganisms.

I  Aerobic Plate Count

The tablet colony counting method is an effective method for counting the number of bacteria. Method is as follows: the sample under test after appropriate dilution, which is fully dispersed into a single cell of microorganism, take a certain amount of sample dilution liquid coating to the tablet, after training, each single cell grows and forms colonies visible to the naked eye, that is, a single colony shall represent a single cell in the original sample; The number of bacteria in the sample can be calculated according to its dilution and inoculation quantity.

II  Yeast and Mold Count

Mold and yeast can also cause food decaying. As their slow growth and competition ability is not strong, so they often appear in the food which is not suitable for the growth of the bacteria, and the food can be low pH, low humidity and high salt and sugar food, low temperature storage of food, food containing antibiotics, etc. So mold and yeast are also used as indicators of food hygiene. The count of mold and yeast refers to the number of fungi and yeast colony in 1g or 1ml samples after the treatment of food samples.

III  E.coli

E. coli refers to possession of certain characteristics of a set of associated with fecal contamination of bacteria, aerobic and facultative anaerobic, and it can break down lactose in 37 ℃ to produce acid maternity leave, so its detection mainly has three steps: lactose fermentation experiments, cultivation, confirm the test. Alfa Chemistry can provide the E. coil detection method to meet the needs of customers.

IV  Salmonellaspp.

Salmonellosis refers to the general term for different forms of human, domestic and wild animals caused by various types of salmonella. Contamination of food by people infected with salmonella or feces of the carrier can cause food poisoning. Salmonella is a common food-borne pathogen. The traditional methods of salmonella identification are mainly based on morphological characteristics, culture characteristics, physiological and biochemical characteristics, antigen characteristics, phage characteristics, etc.

V  Pseudomonasspp.

Pseudomonas aeruginosa is pseudomonas, which is a straight or slightly curved gram negative bacilli which is a non-nuclear bacterium. The main detection methods for the membrane filter technique, through filters with pore size of 0.45 micron and cultivation, can in brominated ammonium cetyl three selective medium to grow and produce pyocyanin namely confirmed pseudomonas.

VI  Staphylococcusaureus

The bacteria are the main bacteria in the secondary microflora. The poisoning caused by the corruption of dairy products and meat is believed to be caused by such bacteria.The test method is to take the specimen smear, and the microscopic examination after gram staining, according to the bacterial form, arrangement and staining can make a preliminary diagnosis, and then the isolation culture can be tested.