Methods Of Peptide Preservation

Storage of Freeze-dried Peptides

Storage of Peptide Solutions

• Usually the peptide products you receive are freeze-dried powder packaging. After receiving the sample, please immediately store the peptide in a dry, light-proof -20 ℃ freezer to maintain the stability of the peptide as much as possible. If stored below -80 ℃, most peptides can maintain activity for several years.
• The external temperature will not affect the shelf life and quality of the peptide during short-term transportation.
• When using peptide products, please place the packaging tube containing the peptide from the freezer to room temperature dry conditions before opening the lid, and wait for its temperature to naturally rise to room temperature before opening the bottle cap. For products stored at -20 ℃, this process takes one hour or longer, depending on the size of the package. Otherwise, water vapor in the air will enter the sample tube when the lid is opened, reducing the stability of the peptide. Once opened, it should be weighed quickly and sealed immediately to avoid deliquescence, especially hydrophilic peptides should be careful to avoid repeated freezing and thawing.
• For easily oxidized peptides containing Cys, Met, Try and easily degraded peptides containing Gln, Asn, Asp in the sequence, repeated thawing and freezing should be avoided to prevent the peptides from being oxidized and degraded. Therefore, it is recommended to store them in small packages. Thaw only as much as needed, discard after use, and do not store.

Peptide solutions are much less stable than freeze-dried powder. Their preservation usually follows the following principles:

• Dissolve in a sterile buffer of pH 5-7 and store at -20 ℃.
• Peptides containing Cys, Met, Try, and Asp are easily oxidized, so they must be stored in an oxidant-free environment.
• Since bacteria can degrade peptides, they must be filtered and sterilized before storage.