Peptide Fluorescent Labeling

Peptide fluorescent labeling is a valuable technique used in biological research to visualize and track specific peptides within cells and tissues. By attaching fluorescent dyes to peptides, researchers can study the dynamics and interactions of these molecules in real-time. Peptide fluorescent labeling is non-radioactive and the experimental operation is simple. Therefore, peptide fluorescent labeling is currently widely used in biological research. People use fluorescent labeled peptides to detect the activity of target proteins, and also apply them in drug development and diagnosis.

Alfa Chemistry has mature technology in peptide fluorescent labeling and can provide various peptide fluorescent labeling services. Our team of experienced scientists and experts are dedicated to offering high-quality, efficient, and customized fluorescent labeling solutions to support your research goals. From selecting the most suitable fluorescent dyes to optimizing labeling protocols, we offer a range of services to ensure the successful labeling of peptides for your specific applications. Partner with us to benefit from our expertise in peptide fluorescent labeling and access cutting-edge solutions for your research needs.

Our Services

Alfa Chemistry can provide a full range of fluorescent labels, and can label fluorescent dyes on the N-terminus, C-terminus, lysine (Lys) side chain amino group and cysteine (Cys) side chain thiol group of the peptide. Below are some of our commonly used fluorescent markers.

FITC (Fluorescein isothiocyanate)

Name	Ex (nm)	Em (nm)	Color	Markable location
FITC	495	521	Green	N-terminus of peptide, Lys side chain

FAM (Carboxyfluorescein)

Name	Ex (nm)	Em (nm)	Color	Markable location
FAM	492	517	Green	N-terminus of peptide, Lys side chain

Rhodamine B

Name	Ex (nm)	Em (nm)	Color	Markable location
Rhodamine B	543	565	Bright red	N-terminus of peptide, Lys side chain

TAMRA (5-Carboxytetramethyl rhodamine)

Name	Ex (nm)	Em (nm)	Color	Markable location
TAMRA	543	572	Orange	N-terminus of peptide, Lys side chain

Cy5 (1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dienyl)-3H-indolium chloride)

Name	Ex (nm)	Em (nm)	Color	Markable location
Cy5	650	670	red	N-terminus of peptide, Lys side chain

MCA (7-Methoxycoumarin-4-acetic acid)

Name	Ex (nm)	Em (nm)	Color	Markable location
MCA	325	393	Blue purple	N-terminus of peptide, Lys side chain

AMC (7-Amino-4-methylcoumarin)

Name	Ex (nm)	Em (nm)	Color	Markable location
AMC	351	450	Blue	C-terminus of peptide

AFC (7-Amino-4-trifluoromethylcoumarin)

Name	Ex (nm)	Em (nm)	Color	Markable location
AFC	382	480	Blue	C-terminus of peptide

Dansyl (5-(Dimethylamino) naphthalene-1-sulfonyl)

Name	Ex (nm)	Em (nm)	Color	Markable location
Dansyl	342	560	Yellow	N-terminus of peptide

EDANS (5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid)

Name	Ex (nm)	Em (nm)	Color	Markable location
EDANS	340	490	N/A	C-terminus of peptide

In addition to the above commonly used fluorescent labels, we also have the following fluorescent labeling substances for reference.

Name	Ex (nm)	Em (nm)	Name	Ex (nm)	Em (nm)
Hydroxycoumarin	325	386	R-phycoerythrin (PE)	565	578
Dansyl chloride	340	578	Rhodamine Red-X	560	580
ROX	575	602	Dabcyl	453	-
Cy2	490	510	Cy3	550	570
Cy3.5	581	596	Cy5	650	670
Cy5.5	675	694	Cy7	743	767
Texas Red	589	615	Hexachlorofluorescein (HEX)	535	556
TruRed 490	675	695	TRITC	547	572

Why Choose Us

Our expert team is skilled in efficiently and accurately incorporating fluorescent labels into peptides, allowing for enhanced detection, visualization, and tracking in various biological systems.
We offer a wide selection of fluorescent dyes and labels, ensuring flexibility and customization to suit specific project demands.
We adhere to strict quality control measures to ensure that our labeled peptides exhibit maximum stability, brightness, and specificity.
We have cutting-edge technology and state-of-the-art equipment that guarantee precise and reliable results.