Protected Amino Acid Identification

The identification of different protected amino acids is of great significance for studying the structure and function of peptides and proteins. Alfa Chemistry has liquid chromatography, gas chromatography, liquid mass spectrometry and other instruments used for protected amino acid identification, which can identify protected amino acids sensitively, accurately and quickly. We provide customers with a full range of protected amino acid identification services from experimental plan design, identification, data analysis to project completion reports.

What We Offer

Alfa Chemistry provides high-precision and high-accuracy protected amino acid identification services. Typical services include the following aspects.

• Structural identification of protected amino acids
• Component identification of protected amino acids
• Enantiomeric identification of protected amino acids
• Impurity identification

Identification Methods

Alfa Chemistry has extensive experience in the identification of protected amino acids, and we will choose the most appropriate method to provide you with efficient services. Commonly used methods are shown below.

• Chromatography. Chromatography is a simple and commonly used method for identifying protected amino acids. Liquid chromatography dissolves the protected amino acid sample in a solvent, separates it through a chromatographic column, and then detects the absorption peaks of different protected amino acids through a detector to determine its composition and content. In addition, gas chromatography and thin layer chromatography are also simple, fast and sensitive methods for the identification of protected amino acids.
• Mass spectrometry (MS). Mass spectrometry is a highly sensitive method for identifying protected amino acids. By ionizing a protected amino acid sample and then analyzing it with a mass spectrometer, the mass spectrum of the protected amino acid can be obtained. According to the characteristic peaks of the mass spectrum, the molecular weight and structure of the protected amino acid can be determined. Commonly used mass spectrometry techniques include electrospray ionization mass spectrometry (ESI-MS) and time-of-flight mass spectrometry (TOF-MS). These techniques can provide information on the molecular mass and fragment ions of protected amino acids, allowing for identification.
• Nuclear Magnetic Resonance (NMR) Spectroscopy. Nuclear magnetic resonance spectroscopy is a technique based on the resonant absorption of atomic nuclei in a magnetic field. By measuring the resonance signals of specific atomic nucleus in protected amino acids, their chemical structure and molecular environment can be determined, thereby identifying different protected amino acids.
• Acid-base titration. Acid-base titration confirms the identity of protected amino acids by measuring its reaction under different pH conditions. Dissolve the protected amino acid to be tested in water, add acid or alkali and perform titration to observe changes in pH value. According to the acidic and basic properties of the functional groups of the protected amino acids (such as carboxyl and amino groups), the acidic, basic or neutral nature of the protected amino acids can be determined.
• Paper electrophoresis method. Paper electrophoresis is a simple and rapid method for identifying protected amino acids. Through electrophoresis technology, the migration speed and color changes of protected amino acids on paper can be observed to judge their different properties and quantities.
• Other methods. Ultraviolet absorption spectroscopy, acid-base hydrolysis, isotope labeling, etc. are also used for the identification of protected amino acids.

Relying on advanced equipment and experienced technical personnel, Alfa Chemistry can quickly and accurately identify protected amino acids. Your inquiries are welcome.