Analysis And Detection Of Peptides

Peptide analysis and detection play a crucial role in various fields such as drug discovery, proteomics, clinical diagnostics, and biomarker identification. Analyzing and detecting peptides allow researchers to gain insights into the functions, interactions, and structures of these important molecules. Alfa Chemistry provides professional and reliable peptide analysis and detection services. Our expertise in peptide analysis and detection allows us to offer comprehensive services for the characterization and quantification of peptides across various applications in biotechnology, pharmaceuticals, and clinical research.

Our Services

Alfa Chemistry provides a range of peptide analysis and detection services to meet your needs in scientific research. The analysis and detection items we provide are as follows.

Items	Content description
Peptide molecular weight determination	The molecular weight of a peptide is a critical parameter that can provide valuable information about its structure and composition. At Alfa Chemistry, peptide molecular weight determination typically uses mass spectrometry (MS), which is a powerful analytical tool for accurately measuring peptide mass.
Peptide purity determination	We can assess the purity of peptide samples to ensure that they meet the required quality standards for research or therapeutic use. High-performance liquid chromatography (HPLC) and MS are commonly used to quantify impurities and contaminants in peptide samples.
Peptide content determination	Peptide content determination refers to the process of quantifying the amount of peptides present in a sample. The peptide content in the sample can be measured accurately and reliably through amino acid analysis, UV spectroscopy and other methods.
Peptide elemental analysis	Elemental analysis of peptides is very important for understanding their biological functions. Elemental analysis of peptides is typically performed using a variety of analytical techniques such as MS, nuclear magnetic resonance (NMR) spectroscopy, and chromatography. These techniques can help identify the individual amino acids present in the peptide and determine their relative abundance.
Amino acid sequencing	Amino acid sequencing is essential for understanding the structure and function of peptides. We used Edman degradation method, tandem mass spectrometry to complete the sequence determination.
Counterion quantitative analysis	Counter ions, including acetate ions, hydrochloride ions, trifluoroacetate ions, etc., are introduced during the peptide synthesis process and eventually exist in the finished peptide. We have a variety of techniques available for the quantitative analysis of counterions, including ion chromatography, capillary electrophoresis, MS, and titration.
Moisture content analysis	We determined the moisture content in peptide samples using the Karl Fischer Titration method. Determining the moisture content of a peptide sample is important to determine the variation in peptide product content between different batches of the same peptide.
Stability testing	We can evaluate the stability of peptides dissolved in different solvents. The detection method is to dissolve the peptide in a specific solvent and keep it for a certain period of time, detect the dissolution, and provide corresponding HPLC and MS reports.
Specific rotation analysis	Specific rotation analysis is used to determine the optical activity of peptides, especially peptide samples containing chiral amino acids.
Amino acid analysis	Determine the content of amino acids in the sample, which can be used as an indicator of net peptide content. First, we hydrolyze the peptide into independent amino acids under acidic conditions, and then use HPLC to detect the amino acid derivatives and perform UV detection for quantification.

Analysis And Detection Of Peptides

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Service Process