Carrier Protein Conjugation

Carrier protein conjugation is a process used in peptide chemistry to enhance the immunogenicity of peptides by conjugating them to carrier proteins. Peptide-carrier protein conjugation is often used to prepare peptide antibodies. A single peptide is usually too small to stimulate a sufficient immune response, while a carrier protein with many antigenic epitopes is beneficial for stimulating helper T cells and further inducing B cell immune responses. Alfa Chemistry has extensive experience in peptide-carrier protein conjugation. We have successfully delivered a large number of carrier protein-conjugated peptides to our customers.

Our Services

Alfa Chemistry provides a wide range of carrier protein conjugation services. The carrier proteins we use for conjugation are mainly the following:

• Keyhole Limpet Hemocyanin (KLH). KLH also known as hemocyanin, is a large aggregated protein. It is derived from the hemolymph of certain mollusks and arthropods (spiders and beetles). KLH contains two copper ions directly connected to the polypeptide chain. Similar to iron-containing hemoglobin, KLH is easy to bind to oxygen and also easy to dissociate from oxygen. It is the only known copper protein that can reversibly bind to oxygen. KLH is the most commonly used carrier protein due to its high immunogenicity.
• Bovine Serum Albumin (BSA). BSA is a stable and soluble albumin. It has approximately 30-35 primary amino groups available for conjugation with linkers, making BSA a popular carrier protein for weak antigenic compounds.
• Ovalbumin (OVA). OVA is the most abundant protein in egg white. OVA is preferred for its well-characterized immunogenicity profile and the availability of specific antibodies for detection.
• Mollusk-derived hemocyanin (CCH). CCH is a hemocyanin protein sourced from mollusks like Concholepas concholepas. CCH is another immunogenic carrier protein that is used in peptide conjugation for immunization purposes.
• Bovine Thyroglobulin (THY). THY is a glycoprotein found in the thyroid gland of cows and is often used as a carrier protein for peptide conjugation experiments. THY has been used in research studies due to its ability to induce a strong immune response when coupled with peptides.

The types of conjugation we provide are as follows:

Alfa Chemistry offers a wide range of peptide chelation modifications to ensure optimal performance and functionality of the modified peptides. The commonly used chelators we offer are listed in the table below.

Our Advantages

• Diverse conjugation types. We have a variety of carrier protein conjugation types for customers to choose from to meet different downstream applications of customers.
• Rich conjugation experience. Our team have extensive experience in carrier protein conjugation and can select the best conjugation scheme based on the sequence characteristics and downstream applications provided by the customer.
• High conjugation efficiency. We can complete carrier protein conjugation in one to two weeks.
• Strict confidentiality. We strictly keep the customer's peptide sequence information confidential and never disclose any information about the customer's ongoing projects.

Service Process