Protected Amino Acids / Alfa Chemistry
Peptide Stable Isotope Labeling
Peptide Stable Isotope Labeling
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Peptide Stable Isotope Labeling

Stable isotope labeled peptides refer to peptides synthesized with stable isotope labeled amino acids. Compared with standard amino acids, stable isotope labeling refers to replacing the original 1H, 12C and 14N atoms with 2H, 13C or 15N. By introducing stable isotopes into peptides, researchers can track and quantify the abundance of specific peptides in different samples. This allows for the comparison of protein expression levels, post-translational modifications, and interactions in a high-throughput and accurate manner. Alfa Chemistry focuses on providing cutting-edge peptide stable isotope labeling services that cater to the diverse needs of researchers and biopharmaceutical companies across various fields.

Our Services

Alfa Chemistry provides advanced peptide stable isotope labeling services for a wide range of research and development purposes. Our team of skilled professionals utilizes state-of-the-art technologies to enable accurate and reliable labeling of peptides for a wide range of applications. Whether you are looking to analyze protein-protein interactions, study protein expression levels, or conduct quantitative proteomics research, our peptide stable isotope labeling services are tailored to meet your specific requirements. We offer labeling services for the following stable isotopes.

  • 2H labeling
  • 15N labeling
  • 13C labeling
  • 15N and 13C double labeling

The following table shows our commonly used isotope labeled amino acids. Other isotope labeled amino acids can be provided upon request.

Amino acid nameLabel locationMolecular weight differenceIsotope abundance
Alanine / Ala(A)U-13C3, U-15N+4 Da> 99%
Arginine / Arg(R)U-13C6, U-15N4+10 Da> 99%
Arginine / Arg(R)4,4,5,5-13C+4 Da> 98%
Isoleucine / Ile(I)U-13C6, U-15N+7 Da> 99%
Leucine / Leu(L)U-13C6, U-15N+7 Da> 99%
Lysine / Lys(K)U-13C6, U-15N2+8 Da> 99%
Proline / Pro(P)U-13C5, U-15N+6 Da> 99%
Phenyalanine / Phe(F)U-13C9, U-15N+10 Da> 99%
Valine / Val(V)U-13C5, U-15N+6 Da> 99%

Applications Of Stable Isotope Labeled Peptides

  • Functional protein quantification. Stable isotope labeled peptides facilitate accurate and precise quantitation of target proteins within complex biological samples. Leveraging techniques like mass spectrometry (MS) and liquid chromatography-mass spectrometry (LC-MS), these labeled peptides allow for comparative analysis of protein abundance in different conditions or samples.
  • Post-translational modification protein quantification. Stable isotope labeled peptides play a critical role in post-translational modification (PTM) protein quantification, allowing researchers to investigate and quantify various PTMs such as phosphorylation, acetylation, and glycosylation within proteins. By incorporating stable isotope labeled amino acids at specific residues within peptides, researchers can pinpoint and quantify PTMs with exceptional accuracy, shedding light on the intricate regulatory mechanisms governing cellular functions.
  • Protein structure analysis. Stable isotope labeled peptides serve as indispensable tools for elucidating protein folding, dynamics, and interactions. By employing advanced techniques like nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS)-based proteomics, researchers can utilize labeled peptides to probe protein conformational changes, mapping protein-protein interactions, and deciphering the structural architecture of complex protein assemblies.
  • Protein expression monitoring. Stable isotope labeled peptides can also be used to monitor changes in protein expression levels over time, enabling longitudinal studies of protein dynamics and turnover rates. By introducing labeled peptides into cellular systems and tracking their abundance through MS-based quantitation, researchers can gain insights into protein expression kinetics, turnover rates, and degradation pathways, offering a comprehensive view of cellular protein dynamics.

Additionally, stable isotope labeled peptides are also used in pharmacokinetic studies, metabolomics studies, monitoring of clinical biochemical properties of drugs and metabolites, protein interaction analysis, etc.

Service Features

  • Customized to increase MS molecular weight according to customer requirements.
  • Strict quality testing, including MS testing and HPLC testing.
  • 2H, 13C, 15N isotope labels are available.
  • A variety of isotopically labeled amino acids are available.