Peptide methylation is a post-translational modification that involves the addition of methyl groups to the amino acid residues within peptides or proteins. It can occur on a variety of amino acid residues, the most common being lysine and arginine. Methylation modification of peptides can have significant effects on their structure, stability, and function. For example, methylation of lysine residues in histone proteins can regulate gene expression by influencing chromatin structure and DNA accessibility. Methylation of arginine residues in proteins can affect protein-protein interactions and signaling pathways. In order to meet the needs of scientific researchers, Alfa Chemistry has developed peptide methylation modification services based on its own technical advantages to provide assistance in your research on protein post-translational modifications.
Our Services
We provide a range of methylation modification services to meet the diverse needs of our customers. The methylation modifications we specialize in include:
Depending on the type of methylated amino acid, we can provide the following peptide methylation modification services.
- Arginine (Arg) methylation modification
- Lysine (Lys) methylation modification
- Tyrosine (Tyr) methylation modification
- Threonine (Thr) methylation modification
- Serine (Ser) methylation modification
- Cysteine (Cys) methylation modification
- Tryptophan (Trp) methylation modification
- Alanine (Ala) methylation modification
- Valine (Val) methylation modification
Depending on the number of methylation modifications, we can provide the following peptide methylation modification services.
- Lysine (Lys) monomethylation modification
- Lysine (Lys) dimethylation modification
- Lysine (Lys) trimethylation modification
- Arginine (Arg) monomethylation modification
- Arginine (Arg) dimethylation modification
Depending on the location of the methylation modification, we can provide the following peptide methylation modification services.
| Backbone amino N-methylation modification |
| Alanine (Ala) backbone amino N-methylation modification |
| Valine (Val) backbone amino N-methylation modification |
| Tyrosine (Tyr) backbone amino N-methylation modification |
| Lysine (Lys) backbone amino N-methylation modification |
| Side chain group methylation modification |
| Lysine (Lys) side chain monomethylation modification |
| Lysine (Lys) side chain dimethylation modification |
| Lysine (Lys) side chain trimethylation modification |
| Arginine (Arg) side chain monomethylation modification |
| Arginine (Arg) side chain symmetric dimethylation modification |
| Arginine (Arg) side chain asymmetric dimethylation modification |
Advantages of Peptide Methylation Modification
- Enhanced stability. Methylation modification can increase the stability of peptides against enzymatic degradation. This is particularly useful in therapeutic peptides that are prone to rapid breakdown in biological systems.
- Improved binding affinity. The addition of methyl groups can significantly alter the binding affinity of peptides to their target molecules, potentially enhancing their efficacy.
- Increased lipophilicity. Methylation modification can increase the hydrophobicity of peptides, which can be advantageous for cell membrane permeability and potentially better oral bioavailability.
- Modulation of biological activity. Methylation modification can modulate the biological activity of peptides and enhance or inhibit their natural function. This can be harnessed to create more potent or more selective bioactive compounds.
- Conformational changes. Methyl groups can induce conformational changes in peptides, affecting their secondary and tertiary structures. This can be used to stabilize specific conformations necessary for biological activity.
- Selective interaction. Methylation modification can create or eliminate specific recognition sites for protein-protein or protein-DNA interactions, providing a tool for modulating these interactions.
Applications of Methylation Modified Peptides
- Drug development. Methylated peptides can serve as more stable and effective therapeutic agents, especially in the treatment of diseases like cancer, metabolic disorders, and infectious diseases.
- Biological research. Methylated peptides can be used to probe signaling pathways and understand the mechanisms of post-translational modifications.
- Peptide vaccine development. Methylated peptides can be used to design vaccine candidates with improved stability and immunogenicity.
Service Process
