Peptide Biotin Labeling

Biotin, a small molecule consisting of a vitamin B derivative, is attached to peptides to enable their detection and isolation through a variety of methods, such as affinity purification and immunoprecipitation. Biotin labeling technique is highly sensitive, specific, and versatile, making it a valuable tool for studying protein-protein interactions, protein localization, and signal transduction pathways. Alfa Chemistry offer comprehensive peptide biotin labeling services tailored to meet the specific needs of our clients.

Our Services

Alfa Chemistry offers customizable labeling options to accommodate specific peptide sequences and desired labeling density. Usually, peptides are labeled with biotin at the N-terminus of the peptide chain or lysine (Lys) side chain. The C-terminus of the peptide chain can also be labeled with biotin, but a Lys or ethylenediamine structure needs to be introduced. We recommend N-terminal labeling, which has a higher success rate, shorter time, and simpler operation.

At Alfa Chemistry, our team of experienced scientists utilizes state-of-the-art equipment and techniques to ensure precise and efficient labeling processes. Whether you require biotin-labeled peptides for affinity purification, immunoprecipitation, or other applications, our services can be tailored to meet your unique requirements. We are committed to delivering exceptional quality and timely results, helping our clients advance their research and achieve their scientific goals.

Applications Of Biotin Labeled Peptides

Biotin labeled peptides are commonly used in various biochemical and molecular biology applications due to their ability to bind specifically to avidin or streptavidin, which allows for easy detection and purification. Some common applications of biotin labeled peptides include:

• Immunoprecipitation and affinity purification. Biotin labeled peptides can be used to selectively isolate proteins or protein complexes from complex biological samples by immobilizing the biotinylated peptide on streptavidin beads and capturing the target proteins.
• Protein-protein interaction studies. Biotin labeled peptides can be used to study protein-protein interactions by binding to specific target proteins. This can help in understanding the molecular mechanisms underlying various biological processes.
• Cell surface labeling. Biotin labeled peptides can be used to label cell surface proteins for flow cytometry analysis or immunofluorescence microscopy.
• Enzyme-linked immunosorbent assay (ELISA). Biotin labeled peptides can be used as detection reagents in ELISA assays to quantify the amount of a specific protein in a sample.
• Protein microarrays. Biotin labeled peptides can be immobilized on a solid support to create protein microarrays for high-throughput screening of protein-protein interactions or antibody-antigen interactions.
• Drug delivery. Biotin labeled peptides can be conjugated to drugs or nanoparticles for targeted drug delivery to specific cells or tissues. Biotin receptors are overexpressed on many cancer cells, making them potential targets for drug delivery.

Our Advantages

• Experienced team. Our team of researchers have extensive experience in biotin labeling techniques. We bring a deep understanding of the intricacies of peptide labeling and can provide high-quality, reliable peptide biotin labeling services.
• Quality assurance. Quality is of utmost importance to us, and we adhere to stringent quality control measures throughout the biotin labeling process to ensure the reliability of our biotin labeling services.
• Timely delivery. Our efficient workflows enable us to deliver results promptly without compromising on quality.
• Competitive pricing. Our company offers competitive pricing for peptide biotin labeling services, making it accessible to a wide range of clients, from academic researchers to pharmaceutical companies. We strive to provide cost-effective solutions without compromising on the quality of our services.