Common Resins For Peptide Synthesis

In the process of peptide synthesis, resins are widely used as solid phase carriers. The following are several commonly used resins for synthesizing peptides.

Polystyrene Resin

Polystyrene resin is one of the most commonly used resins in peptide synthesis. It is a cross-linked polystyrene polymer with functional groups that can be easily modified to attach the first amino acid of the peptide chain. Polystyrene resin is known for its high loading capacity, which allows for the synthesis of longer peptide chains. However, polystyrene resin can be difficult to swell, leading to slower reaction rates and potentially lower yields.

Polyacrylamide Resin

Polyacrylamide resin is a commonly used solid support for peptide synthesis due to its high swelling capacity, mechanical stability, and chemical compatibility. It consists of a cross-linked polyacrylamide matrix that provides a stable scaffold for peptide bond formation. The resin is typically used in Fmoc-based solid-phase peptide synthesis, where the amino acid residues are sequentially added to the growing peptide chain.

Polyethylene Glycol Resin

Polyethylene glycol resin, also known as PEG resin, is a versatile solid support for peptide synthesis that offers several advantages, including good swelling properties, high chemical stability, and compatibility with a wide range of solvents. PEG resin is particularly useful for the synthesis of hydrophobic peptides or peptide libraries, as it can improve solubility and increase the overall yield of the desired peptide. The resin can be functionalized with various linker molecules to enable efficient peptide coupling and subsequent cleavage from the resin.

Polyethyleneimine Resin

Polyethyleneimine resin is a cross-linked polymer matrix containing polyethyleneimine groups. This resin offers good loading capacity, mechanical stability, and chemical compatibility, making it suitable for the efficient synthesis of peptides with a wide range of sequences and modifications. Polyethyleneimine resin is often used in Boc-based solid-phase peptide synthesis, where the amino acid residues are sequentially added to the resin-bound peptide chain.

TentaGel Resin

TentaGel resin is a polymer resin with a polyethylene glycol (PEG) backbone, which provides better solubility and swelling properties compared to polystyrene resin. TentaGel resin is known for its high mechanical stability and compatibility with a wide range of chemical reactions. However, TentaGel resin typically has a lower loading capacity compared to polystyrene resin.

Wang Resin

Wang resin is a popular resin choice for the synthesis of C-terminal amide peptides. It is a resin with a linker that allows for the cleavage of the peptide from the resin with simultaneous formation of an amide bond at the C-terminus. Wang resin is known for its ease of use and compatibility with a variety of peptide coupling agents. However, it may not be suitable for the synthesis of longer peptide chains due to its lower loading capacity compared to other resins.

4-Methylbenzhydrylamine Resin

4-Methylbenzhydrylamine resin, also known as MBHA resin, has good swelling properties, chemical stability, and compatibility with common peptide coupling reagents. The resin contains a benzhydrylamine linker that allows for efficient peptide bond formation. MBHA resin is commonly used in Fmoc-based solid-phase peptide synthesis and enables the synthesis of complex peptides with high purity and yield.

Aminomethylresin

Aminomethylresin, or AM resin, is featured by a polymer matrix functionalized with aminomethyl groups for efficient peptide coupling. The resin offers good swelling properties, chemical stability, and compatibility with a variety of peptide synthesis reagents and conditions. AM resin is frequently employed in Boc-based solid-phase peptide synthesis, where the amino acid residues are added sequentially to the growing peptide chain. AM resin is suitable for the synthesis of a wide range of peptides, including those with complex structures or modifications.