Peptide FRET Labeling

FRET (fluorescence resonance energy transfer) is a distance-dependent process in which the excited state energy of a donor molecule is transferred to an acceptor molecule, resulting in fluorescence quenching. This technique can detect molecular interactions in the nanometer range. The dye molecules used in FRET experiments can be the same, but in most cases the donor molecule (fluorophore) and acceptor molecule (quencher group) are two different dye molecules. By labeling peptides with fluorescent dyes that can transfer energy between each other, researchers can monitor changes in distance and orientation between the labeled peptides, providing a sensitive and quantitative measurement of molecular interactions. Alfa Chemistry has extensive experience in peptide FRET labeling, and we can provide a full range of FRET fluorescence combination pairs for your scientific research.

FRET Technology

FRET is a powerful and widely used technique in molecular and cell biology for studying molecular interactions, conformational changes, and distances between biomolecules. It involves the non-radiative transfer of energy from an excited donor fluorophore to an acceptor fluorophore in close proximity, typically within 1-10 nm range, leading to the emission of light at a longer wavelength by the acceptor. This transfer of energy is highly sensitive to the distance and orientation between the donor and acceptor molecules, making FRET an invaluable tool for studying protein-protein interactions, protein conformation changes, DNA hybridization, and many other biological processes at the molecular level. FRET can be used to quantify distances between molecules, monitor real-time molecular interactions, and provide critical insights into complex biological phenomena. The intrinsic properties of FRET make it a versatile technique with various applications in drug discovery, molecular imaging, fluorescence microscopy, and biosensor development.

Our Services

We can provide high-quality FRET labeling services for peptides, enabling our clients to gain valuable insights into biomolecular interactions with exceptional precision and sensitivity. Below are some of our commonly used FRET fluorescence combination pairs.

Commonly used FRET fluorescence combination pairs

Donor			Acceptor
Name	Ex (nm)	Em (nm)	Name	Ex (nm)	Em (nm)
Cy2	490	510	Cy3	555	570
FITC	494	521	TRITC	557	576
FAM	495	520	Cy3	555	570
FAM	495	520	Texas Red	589	615
FAM	495	520	Cy5	646	662
Cy3	555	570	Cy5	646	662
EDANS	335	493	DABCYL	453	-
MCA	328	393	DNP	348	-
Abz	330	420	DNP	348	-

Why Choose Us

Professional team. Our team consists of experts with extensive experience in FRET technology and peptide labeling, ensuring that your samples are handled with the highest level of processing.
Comprehensive service. We offer a wide selection of FRET fluorescence combination pairs, ensuring flexibility and customization to suit specific project demands.
Quality assurance. Quality is of utmost importance to us, and we adhere to stringent quality control measures throughout the labeling process to ensure the reliability of our FRET labeling services.
Timely delivery. Our efficient workflows enable us to deliver results promptly without compromising on quality.

With our commitment to excellence and a track record of successful collaborations, we are confident in our ability to meet and exceed your expectations for peptide FRET labeling services. Contact us today to discuss how we can support your research goals and contribute to the success of your projects.