Protected Amino Acids / Alfa Chemistry
Protected Amino Acid Separation
Protected Amino Acid Separation
Online Inquiry
Verification code

Protected Amino Acid Separation

Protected amino acid separation has a wide range of applications in the fields of bioscience, including protein structure and function research, drug development and production, food analysis and quality control, etc. In addition, in peptide synthesis methods, whether liquid phase synthesis, solid phase synthesis, or fragment ligation, protected amino acids are used as raw materials. The optical purity of protected amino acids is particularly important to the quality of synthesized peptides, so chiral separation of protected amino acids is required. Protected amino acid isomers also affect the biological activity of peptides, so it is also necessary to separate protected amino acid isomers. Alfa Chemistry has a number of new technologies in the field of protected amino acid separation, and we will provide you with high-quality protected amino acid separation services.

What We Offer

Alfa Chemistry has advanced technology and equipment in the protected amino acid separation process and can provide customers with a variety of protected amino acid separation services. Typical services include the following aspects. If you have other needs, please contact us for more service details.

  • Group separation of protected amino acid mixtures
  • Single component separation of protected amino acid mixtures
  • Chiral separation of protected amino acids
  • Separation of protected amino acid isomers

Separation Methods

Alfa Chemistry masters fast, simple, economical and widely applicable methods for the separation of protected amino acids. We will choose the most appropriate method to provide you with efficient services. Commonly used methods are shown below.

  • Ion exchange chromatography. Ion exchange chromatography is a commonly used separation method for protected amino acids. The principle is to use ion exchange resin to separate protected amino acids. There are certain fixed anions and cations on the resin. When the protected amino acid solution passes through the resin, the anions and cations on the resin adsorb and desorb with the charged groups in the protected amino acids, thereby achieving the separation of the protected amino acids.
  • High performance liquid chromatography (HPLC). HPLC is a method for separation based on the difference in partition coefficients of substances in a solution between the stationary phase and the mobile phase. In HPLC, the appropriate stationary phase and mobile phase can be selected. By adjusting the properties and flow rate of the mobile phase, rapid, accurate and sensitive separation of protected amino acids can be achieved.
  • Capillary electrophoresis method. Capillary electrophoresis is a method for separation based on differences in the migration rates of protected amino acids in capillaries under the action of an electric field. In the capillary electrophoresis method, when the protected amino acid solution passes through the capillary, it is affected by the electric field force. Different protected amino acids migrate at different rates in the capillary according to their charge size and molecular size, thereby achieving the separation of protected amino acids.
  • Gel filtration method. Gel filtration is a method for separation based on molecular size differences of protected amino acids in the pores of the gel. In the gel filtration method, when the protected amino acid solution passes through the gel, due to the size limit of the gel pores, the protected amino acids with larger molecules cannot pass through the gel pores, while the protected amino acids with smaller molecules can pass through the gel pores, thus achieving the separation of protected amino acids.
  • Other methods. Gas chromatography, paper chromatography, isoelectric point precipitation, special reagent precipitation, solvent extraction, reverse micelle extraction, adsorption method, etc. are also used for the separation of protected amino acids.