siRNA Development

Small interfering RNAs (siRNAs), also known as short interfering RNAs or silencing RNAs, are a class of double-stranded RNAs that are at first non-coding RNA molecules, typically 20-24 (normally 21) base pairs in length, and operate within the RNA interference (RNAi) pathway. They interfere with the expression of specific genes with complementary nucleotide sequences by degrading mRNA after transcription, and thus preventing translation. Because of specific and gene silencing, siRNAs are expected to become an important approach for the treatment of a variety of infectious, hematologic tumor, cardiovascular, and neurodegenerative conditions.

Alfa Chemistry provides professional, rapid and high-quality therapeutic siRNA development services at competitive prices for global customers. If you need help, please contact us.

Mechanism of Action of siRNAs

The mechanism of action of siRNAs is described below ^[1]:

Generally, siRNAs exert their effect at the post-transcriptional stage. A siRNA duplex arises when long dsRNA is cleaved by Dicer, a member of the RNAse III family. The duplex is comprised of a passenger strand (sense strand) and a guide strand (antisense strand). These siRNAs get incorporated into the RNA-induced silencing complex (RISC) where it interacts with its Argouate 2 component, resulting in duplex unwinding and degradation of the passenger strand. The guide strand, which is complementary to the target mRNA, then guides the RISC complex to bind the mRNA. In RISC complexes, siRNA and mRNA are transposed, and an endonuclease cuts mRNA into small fragments, specifically inhibiting gene expression.

Our Development Process

Alfa Chemistry provides therapeutic siRNA development services, and the following is our main development process. No matter which stage of siRNA development you encounter challenges, we will provide you with timely help.

• Target selection.
• Sequence design of siRNAs.
• Synthesis and chemical modification of siRNAs.
• Purification and analysis of siRNAs.
• Delivery system selection or development of siRNAs.
• Nonclinical evaluation of siRNAs.
• Scale-up of siRNAs.

Our Advantages

Alfa Chemistry has been developing therapeutic siRNA for many years. Our advantages are mainly as follows:

• Powerful software and algorithms.
• Familiar with siRNA design rules.
• Reliable high-throughput screening technology.
• Experienced experts in biology and pharmacy.
• High sensitivity detection system.
• Efficient data processing system.
• Comprehensive assessment tools.

Our products and services are for research use only and cannot be used for any clinical purposes.

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