Pharmacodynamic Study

Pharmacodynamics (sometimes described as what a drug does to the body) is the study of the biochemical, physiologic, and molecular effects of drugs on the body, involving receptor binding (including receptor sensitivity), post-receptor effects, and chemical interactions. Concretely, pharmacodynamics is the study of how a drug affects an organism, whereas pharmacokinetics is the study of how the organism affects the drug. The pharmacodynamic study is an important part of the oligonucleotide therapeutics development process and provides data to support the clinical application of oligonucleotide therapeutics.

In vitro pharmacodynamics

In vitro pharmacodynamic assays, primary cells, normal cells and transfected cells of animals or humans can be selected to investigate the effects of oligonucleotide therapeutics on cell activity, functional proteins and gene mRNA levels. Since the target of oligonucleotide action is specific mRNA, it is necessary to carry out target binding assays in vitro. This can not only verify the effect on the target, but also provide a reasonable basis for the selection of animal species for toxicological research.