miRNA Development

MicroRNAs (miRNAs) are small non-coding regions located in 20-22 nucleotide RNAs, which play an important role in all biological pathways in multicellular organisms including mammals. A single miRNA can target hundreds of mRNAs and influence the expression of many genes involved in a functional interacting pathway^[1]. In the more than two decades since the discovery of the first miRNA, the field of miRNA biology has made great strides. Insights into the roles of miRNAs in development and disease, particularly in cancer, have made miRNAs attractive tools and targets for novel therapeutic approaches.

With reliable technologies and tools, Alfa Chemistry has the ability to provide customers with therapeutic miRNA development services. We look forward to working with you for the benefit of human health.

Mechanism of Action of miRNAs

The mechanism of action of miRNAs includes several steps, and involves several proteins^[2]. MiRNAs bind to their targets primarily at the untranslated region 3' (UTR). Alternatively, miRNAs also can bind to other regions, such as 5' (UTR), or even to promoter regions in the target mRNA. Binding sites determine the biological consequences of these interactions. Most miRNAs inhibit gene expression by hindering the translation of the target mRNA protein. It is important to note that miRNA binding to the promoter region of a gene may have the opposite effect, namely enhancing gene expression.

Applications of miRNAs

The majority of miRNAs are intracellular, but some miRNAs exist in the extracellular compartment and are considered to be mediators of cell-cell communication. Extracellular miRNAs can be isolated from body fluids such as serum, plasma and saliva. They act as potential biomarkers for the detection of various diseases. In addition, many pharmaceutic companies have recently started investing in developing miRNAs for the treatment of human diseases. For example, Santaris Pharmaceuticals, Rosetta Genomics, and Regulus Therapeutics are focused on developing for liver cancer treatments, Mirna Therapeutics is focused on lung, prostate, and blood cancers, as well as miRagen Therapeutics is focused on cardiovascular and muscle diseases^[3].

Our Development Process

Alfa Chemistry provides therapeutic miRNA development services, and the following is our main development process. No matter which stage of miRNA development you encounter challenges, we will provide you with timely help.

• Target selection.
• Sequence design of miRNAs.
• Synthesis and chemical modification of miRNAs.
• Purification and analysis of miRNAs.
• Delivery system selection or development of miRNAs.
• Nonclinical evaluation of miRNAs.
• Scale-up of miRNAs.

Our products and services are for research use only and cannot be used for any clinical purposes.

Online Inquiry

* Name

Phone

* Email

* Services & Products of Interest miRNA Development

Project Description

* Verification Code

Submit