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absorption spectroscopy (AAS, GFA, FIAS), Our goal is to provide test services as efficiently
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Note: this service is for Research Use Only and Not intended for clinical use.
Biopharmaceutical typically encompasses recombinant therapeutic proteins (including antibodies), nucleic acid-based products, engineering cell-based or tissue-based products. Most of these products are recombinant or engineered versions of natural eukaryotic extracellular proteins so that they will be affected by post-translational modifications1. Protein post-translational modification(PTM) can modify the physical and chemical properties of a protein by affecting the physical and chemical properties of the protein by adding a modifying group to one or more amino acid residues, thereby affecting the protein's spatial conformation, activity, protein folding, and protein-protein interaction.
The most commonly used PTMs associated with licensed therapeutic proteins include carboxylation and hydroxylation, amidation and sulfation, disulfide bond formation and proteolytic processing, and glycosylation. Among them, glycosylation is the most widespread and complex, and it is the PTM most significant related to biopharmaceutical products. Since the molecular mass changes correspondingly after the protein changes and the PTM protein has a low content in the sample and a wide dynamic range, the modified protein or peptide is first enriched and then detected using a highly accurate mass spectrometer. Alfa Chemistry's experts have a deep understanding of PTM and combine advanced technology platforms to provide customized PTM services to meet the diverse needs of customers around the world. Please feel free to contact us for any PTM service needs.
Alfa Chemistry's PTM analysis services include but not limited to:
Acetylation
Alkylation
Methylation
Biotinylation
Glycosylation
Phosphorylation
Sulfation
Ubiquitination
Technical process of protein post-translational modification:
Alfa Chemistry's advantages:
Short Detection Cycle
Competitive Price
Experienced Team of Experts
First-class Equipment
Reliable Results
Familiarity with Various Standards
Reference
GaryWalsh. (2010) 'Post-translational modifications of protein biopharmaceuticals.' Drug Discovery Today. 15(17 -18): 773 - 780.