Food Amino Acid Testing Service

Amino acids are the basic building blocks of proteins. Accurate measurement of amino acid content helps correct food nutrition label analysis to ensure food quality.

Our experts can provide you with comprehensive food amino acid testing service. We strictly abide by the quality assurance/quality control plans and regulatory requirements of each country and region to provide you with support. Including but not limited to the following standards and methods: ISO, FDA/BAM, ASTM, AOAC, EU regulations, LFGB, DGCCRF, DM 21/03/1973, JFSL, GB/T and SN.

Food Amino Acid Testing at Alfa Chemistry

1. Can detect 20 different amino acids, including glycine, alanine, valine, leucine, isoleucine, phenylalanine, proline helic acid, serine, tyrosine, threonine, aspartic acid, glutamic acid, lysine, arginine, histidine, cystine, tryptophan, glutamine, asparagine, taurine, etc.
2. Detection of free amino acids
3. Detection of total amino acids (free and natural)
4. Detect the presence or absence of amino acid in unknown sample
5. Detect the type of amino acid in unknown sample
6. Isolate amino acid from unknown sample
7. Monitor the progress of reactions

Food Amino Acid Testing Platform

• Ninhydrin post-column derivatization method

The cation exchange resin is used as the stationary phase and the acid buffer is used as the mobile phase. The sample undergoes high-efficiency cation exchange chromatography. Ninhydrin is added to the post-column effluent to indirectly detect derivatives with visible light absorption generated by amino acids. Photoamine and o-phthalaldehyde can replace ninhydrin to increase the sensitivity by 3 orders of magnitude. This method is not suitable for proline detection.

• Pre-column derivatization reversed-phase high performance liquid chromatography

Choose a suitable derivatizing agent and quantitatively react with each amino acid to produce a specific product with a certain stability, and then analyze the product by high performance liquid chromatography to reverse the amino acid.

• Thin layer chromatography
• Paper chromatography
• Near infrared spectroscopy

This method has the advantages of fast speed (all analysis results can be obtained in 1 min), no pollution (no chemical reagent discharge), no sample damage, good experiment reproducibility (no-man error), and high accuracy. It can detect free amino acids as well as bound amino acids.

Not suitable for small batch sample analysis. The detection range of this technology cannot completely cover twenty standard amino acids. The scope of coverage depends on the nature of the solution during sample pretreatment and the nature of the amino acid itself (mostly depending on the side chain).

Acceptable Samples: Nearly all raw and processed foods or ingredients.

At Alfa Chemistry, our analytical scientists are experienced in food amino acid testing. After communicating with you about the testing scheme, we will implement it within a quick turnaround time and provide you with an analysis report. If you have any questions or specific needs, do not hesitate to contact us.