CAS 17372-87-1 Eosin Y disodium salt, dye content 85%, indicator

Synonyms

Eosine YB; Phlox Red Toner X-1354; MCULE-3311428648; Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 2',4',5',7'-tetrabromo-3',6'-dihydroxy-, sodium salt (1:2); CHEBI:52053; I14-18436; MFCD00036189; Eosine J; 7549-EP2277567A1; Certiqual Eosine;

IUPAC Name

disodium;2-(2,4,5,7-tetrabromo-3-oxido-6-oxoxanthen-9-yl)benzoate;

Molecular Weight

691.859g/mol

Molecular Formula

C20H6Br4Na2O5;

Canonical SMILES

C1=CC=C(C(=C1)C2=C3C=C(C(=O)C(=C3OC4=C(C(=C(C=C24)Br)[O-])Br)Br)Br)C(=O)[O-].[Na+].[Na+];

InChI

InChI=1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2;

InChI Key

SEACYXSIPDVVMV-UHFFFAOYSA-L;

Melting Point

572 ° F (decomposes) (NTP, 1992);

Solubility

greater than or equal to 100 mg/mL at 70° F (NTP, 1992);

Storage

Store at RT.

Complexity

797

Covalently-Bonded Unit Count

EC Number

241-409-6

Exact Mass

691.67g/mol

H-Bond Acceptor

Heavy Atom Count

Monoisotopic Mass

687.674g/mol

Rotatable Bond Count

Topological Polar Surface Area

89.5A^2

UNII

TDQ283MPCW

Application

Eosin Y disodium salt, with a dye content of 85%, serves multiple purposes in the field of histology and biochemistry. Primarily, it is used as a counterstain to haematoxylin in the widely adopted H&E (haematoxylin and eosin) staining technique, one of the most common methods for examining tissue samples. This brownish-red powder effectively stains cellular structures such as muscle fibers, cytoplasm, and collagen, highlighting them for detailed microscopic analysis. Additionally, Eosin Y plays a crucial role in biochemical applications; it modulates the activity of Mg2+-ATPase by altering its affinity for ATP and Mg2+ at specific concentrations, with noted effects at levels ranging from 10 to 50 µM and changes observed near 100 µM. Beyond its staining and enzyme interaction capabilities, the compound functions as a photosensitizer, facilitating visible-light-driven electron-transfer reactions as a molecular photoelectrode. Eosin Y also enhances fluorescent quenching properties by forming complexes with polyvinylpyrrolidone, thus expanding its utility across various scientific disciplines.

February 12, 2025

Reliable Staining with Eosin Y Disodium Salt

I used Eosin Y disodium salt in my histology research for H&E staining. It effectively counterstained tissues, highlighting cell structures like muscle and collagen. The dye's consistency and vivid coloring were impressive, enhancing my microscopy results.